Influenta alimentatiei asupra parametrilor biochimici la animalele de experienta

UMF Carol Davila Bucuresti

Facultatea de Farmacie

Influenta alimentatiei asupra parametrilor biochimici la animalele de experienta

CUPRINS

PARTEA GENERALA

Capitolul 1.          Introducere

Capitolul 2.          Nutritia animalelor de experienta

Definitia nutritiei. Trofine.

Metabolismul animalului de experienta

Experienta curenta

Standarde

Tendinte

Dieta pregatitoare in studii specifice

testarea efectului asupra glicemiei

testarea efectului asupra profilului lipidic

testarea influentelor hepatotoxice

testarea  efectului imunostimulator

Capitolul 3.          Parametri biochimici investigati

Glicemia

Profil lipidic

lipide totale

colesterol total

fractiile colesterolului

Proteinograma

Teste functionale hepatice

GOT GPT

sinteza proteica

Teste hematologice

PARTEA EXPERIMENTALA

Capitolul 4.          Introducere

Capitolul 5.          Material si Metoda

Capitolul 6.          Rezultate

Capitolul 7.          Discutii

Capitolul 8.          Concluzii

CONCLUZII GENERALE

BIBLIOGRAFIE

PARTEA GENERALA

Capitolul 1.          Introducere

Alimentatia animalelor de experienta este un capitol destinat in mod traditional veterinarilor si tehnicienilor din laboratoarele de cercetare. Este necesara implicarea conducatorilor de proiecte de cercetare in acest aspect deoarece alimentatia influenteaza direct nivelele anumitor parametri biochimici.

Este necesara planificarea atenta a proiectelor de cercetare incepand de la alimentatia si conditiile de viata a animalelor incluse in studiu pentru a avea o acuratete maxima a rezultatelor experimentelor.

Animal Care and Use Protocols

The following topics should be considered in the preparation and review of animal care and use protocols:

• Rationale and purpose of the proposed use of animals.
• Justification of the species and number of animals requested. Whenever possible, the number of animals requested should be justified statistically.
• Availability or appropriateness of the use of less-invasive procedures, other species, isolated organ preparation, cell or tissue culture, or computer simulation (see Appendix A, 'Alternatives').
• Adequacy of training and experience of personnel in the procedures used.
• Unusual housing and husbandry requirements.
• Appropriate sedation, analgesia, and anesthesia. (Scales of pain or invasiveness might aid in the preparation and review of protocols; see Appendix A, 'Anesthesia, Pain and Surgery.')
• Unnecessary duplication of experiments.
• Conduct of multiple major operative procedures.
• Criteria and process for timely intervention, removal of animals from a study, or euthanasia if painful or stressful outcomes are anticipated.
• Postprocedure care.
• Method of euthanasia or disposition of animal.
• Safety of working environment for personnel.

Occasionally, protocols include procedures that have not been previously encountered or that have the potential to cause pain or distress that cannot be reliably controlled. Such procedures might include physical restraint, multiple major survival surgery, food or fluid restriction, use of adjuvants, use of death as an end point, use of noxious stimuli, skin or corneal irritancy testing, allowance of excessive tumor burden, intracardiac or orbital-sinus blood sampling, or the use of abnormal environmental conditions. Relevant objective information regarding the procedures and the purpose of the study should be sought from the literature, veterinarians, investigators, and others knowledgeable about the effects on animals. If little is known regarding a specific procedure, limited pilot studies designed to assess the effects of the procedure on the animals, conducted under IACUC oversight, might be appropriate. General guidelines for evaluation https://www.nap.edu/readingroom/books/labrats/chaps.html#b

Animals should be fed palatable, noncontaminated, and nutritionally adequate food daily or according to their particular requirements unless the protocol in which they are being used requires otherwise. Subcommittees of the National Research Council Committee on Animal Nutrition have prepared comprehensive treatments of the nutrient requirements of laboratory animals (NRC 1977, 1978, 1981a,b, 1982, 1983, 1984, 1985a,b, 1986, 1988, 1989a,b, 1994, 1995). Their publications consider issues of quality assurance, freedom from chemical or microbial contaminants and presence of natural toxicants in feedstuffs, bioavailability of nutrients in feeds, and palatability.

Animal-colony managers should be judicious in purchasing, transporting, storing, and handling food to minimize the introduction of diseases, parasites, potential disease vectors (e.g., insects and other vermin), and chemical contaminants into animal colonies. Purchasers are encouraged to consider manufacturers'

Capitolul 2.          Nutritia animalelor de experienta

Definitia nutritiei. Trofine. - cartea de Igiena Alimentatiei

Proteine

Lipide

Glucide

Vitamine

Microelemente

Apa

Metabolismul animalului de experienta - web site

Experienta curenta - d-na Prof.

Standarde -

https://www.labdiet.com

Tendinte https://www.researchdiets.com/OSD/formulas/index.htm , https://www.researchdiets.com/OSD/diets/test_comp.htm

Dieta pregatitoare in studii specifice

- Diet- Induced Disease Models - intri pe link si dai tabele cu dieta de aici

testarea efectului asupra glicemiei>Diabetes

testarea efectului asupra profilului lipidic

>Obesity

>Metabolic Syndrome

>Atherosclerosis

https://www.researchdiets.com/pdf/Data%20Sheets/DIO%20Series.pdf

Formulated by E. A. Ulman, Ph.D., Research Diets, Inc., 8/26/98 and 3/11/99.

*Typical analysis of cholesterol in lard = 0.95 mg/gram.

D12450B - Cholesterol (mg)/4057 kcal = 19 , Cholesterol (mg)/kg = 18

D12451 - Cholesterol (mg)/4057 kcal = 168.6, Cholesterol (mg)/kg = 196.5

D12492 - Cholesterol (mg)/4057 kcal = 232.8 Cholesterol (mg)/kg = 300.8

testarea influentelor hepatotoxice (

testarea efectului imunostimulator (?)

>Hypertension

>Cancer

>Osteoporosis

AIN-76 SEMIPURIFIED DIET (Cat.# 905452) Rat or Mouse Powdered For complete diet composition, please see the Animal Research Diets Section of this catalog.

AIN-76 SEMIPURIFIED DIET (Cat.# 905453) Rat or Mouse Pelleted For complete diet formulation, please see the Animal Research Diets Section of this catalog.

AIN 76-A SEMIPURIFIED DIET (Cat.# 960096) Rat or Mouse Powdered AIN-76 diet with the amount of Vitamin K increased 10-fold (to 500 g/Kg of diet). For complete diet composition, please see the Animal Research Diets Section of this catalog.

AIN 76-A SEMIPURIFIED DIET (Cat.# 960097) Rat or Mouse Pelleted AIN-76 diet with the amount of Vitamin K increased 10-fold (to 500 g/Kg of diet). For complete diet composition, please see the Animal Research Diets Section of this catalog.

AIN-76 B-40 DIET (Cat.# 960353) Rat or Mouse Powdered AIN-76A diet with 40% corn starch and sucrose. For complete diet composition, please see the Animal Research Diets Section of this catalog.

AIN-76 B-40 DIET (Cat.# 960354) Rat or Mouse Pelleted AIN-76A diet with 40% corn starch and sucrose. For complete diet composition, please see the Animal Research Diets Section of this catalog.

AIN-76 B-65 DIET (Cat.# 960355) Rat or Mouse Powdered AIN-76A diet with 65% corn starch (not available in pelleted form). For complete diet composition, please see the Animal Research Diets Section of this catalog.

AIN-76C SEMIPURIFIED DIET POW DERED (Cat.# 960295) Rat or Mouse Powdered This diet contains 500 g/Kg menadione sodium bisulfite and BHT 0.02%/g of oil. For complete diet composition, please see AIN-76 Diet in the Animal Research Diets Section of this catalog.

AIN-76C SEMIPURIFIED DIET PEL LETED (Cat.# 960296) Rat or Mouse Pelleted AIN-76 diet with 500 g/Kg menadione sodium bisulfite and 0.02% BHT/g of oil. For complete diet composition, please see the Animal Research Diets Section of this catalog.

AIN-76 D-40 DIET (Cat.# 960356) Rat or Mouse Powdered AIN-76C diet with 40% corn starch and 25% sucrose. For complete diet composition, please see AIN-76 diet in the Animal Research Diets Section of this catalog.

AIN-76D-40 DIET (Cat.# 960357) Rat or Mouse Pelleted AIN-76C diet with 40% corn starch and 25% sucrose. For complete diet composition, please see AIN-76 diet in the Animal Research Diets Section of this catalog.

AIN-76 D-65 DIET (Cat.# 960358) Rat or Mouse, wdered,AIN-76C diet with 65% corn starch. (Not available in pellet form). For complete diet composition, please see AIN-76 diet in the Animal Research Diets Section of this catalog.

AIN-93 G DIET (Cat.# 960398) PowderedFor growth, pregnancy and lactational phases of rodents For complete diet composition, please see the Animal Research Diets Section of this catalog.

AIN-93 G DIET (Cat.# 960399) PelletedFor growth, pregnancy and lactational phases of rodents For complete diet composition, please see the Animal Research Diets Section of this catalog.

AIN - 93 G - MX MINERAL MIX (Cat.# 960400) Powdered For growth, pregnancy and lactational phases of rodents Not available in pellet form. For complete diet composition, please see the Animal Research Diets Section of this catalog.

AIN-93 M DIET (Cat.# 960396) PowderedFor maintenance of adult rodents This diet is a modification of AIN-76A. For complete diet composition, please see the Animal Research Diets Section of this catalog.

AIN-93 M DIET (Cat.# 960397) PelletedFor maintenance of adult rodents This diet is a modification of AIN-76A, however, due to the addition of dextrinized corn starch replacing most of the sucrose in the AIN-76 diet, the pellets will be small. For complete diet composition, please see the Animal Research Diets Section of this catalog.

AIN - 93 M - MX MINERAL MIX (Cat.# 960401) Powdered For maintenance of adult rodents Not available in pellet form. For complete diet composition, please see the Animal Research Diets Section of this catalog.

AIN - 93 - VX VITAMIN MIX (Cat.# 960402) Powdered Not available in pellet form. For complete diet composition, please see the Animal Research Diets Section of this catalog.

AMINO ACID DIET, SYNTHETIC MODIFIED (Cat.# 960362) Modified Pelleted For complete diet composition, please see the Animal Research Diets Section of this catalog.

Capitolul 3.          Parametri biochimici investigati - aici dai din cartea de Biochimie clinica

Glicemia

Profil lipidic

lipide totale

colesterol total

fractiile colesterolului

Proteinograma

Teste functionale hepatice

GOT GPT

sinteza proteica

Teste hematologice

Capitolul 4.          PARTEA EXPERIMENTALA

Introducere

Alimentatia animalelor de experienta este un capitol destinat in mod traditional veterinarilor si tehnicienilor din laboratoarele de cercetare. Este necesara implicarea conducatorilor de proiecte de cercetare in acest aspect deoarece alimentatia influenteaza direct nivelele anumitor parametri biochimici.

Scopul prezentului studiu este investigarea modificarii parametrilor biochimici glicemia, profilul lipidic si testele functionale hepatice - in functie de tipul de alimente administrate in laborator: nutret combinat granulat pentru sobolani, paine si lapte, vegetale si cereale.

Material si Metoda

Lot II alimentatia a fost asigurata cu nutret combinat granulat, furaj complet pentru soareci, sobolani si hamsteri utilizati in scopuri stiintifice sau experimentale, fabricate la Institutul Cantacuzino din Bucuresti - statiunea Baneasa.

Acestea se administreaza ca atare, la discretie sau confor ratiei, conform prospectului. Ingrediente: cereale, srot soia, srot floarea soarelui, carbonat de calciu, fosfat de calciu, sare, aminoacizi, premix vitamino-mineral. Srotul de soia este obtinut din soia modificata genetic. Proteina bruta: max. 12%, Grasime bruta - min 1,5%, fibre brute - min. 5% - de sunat la 0212694091 pentru a afla ponderea procentuala exacta a materiilor prime.

Rezultate

Discutii

Concluzii

Capitolul 5.          CONCLUZII GENERALE

BIBLIOGRAFIE